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tie inverted light microscope  (Nikon)


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  • 99

    Structured Review

    Nikon tie inverted light microscope
    Tie Inverted Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tie+inverted+light+microscope/Inverted+Microscopes/pm41216790-100-8-7
    Average 99 stars, based on 14758 article reviews
    tie inverted light microscope - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Live Cell Imaging:

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions reveals a novel protrusion length-matching mechanism
    Article Snippet: The tissue sections and fixed cells were imaged using a Nikon A1R laser-scanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488- and 561-nm excitation LASERS, a 100×/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. The membranes were blocked for 1 h in 5% milk in PBS containing 0.1% Tween 20 (PBS-T), washed once with PBS-T, and then incubated with primary antibodies against MYO7B (1:100; Sigma catalog no. HPA039131), CDHR2 (1:100; Sigma catalog no. HPA012569), CDHR5 (1:500; Sigma catalog no. HPA009081), ANKS4B (1:200; Sigma catalog no. HPA043523), USH1C (1:250; Sigma catalog no. HPA027398), or GAPDH (1:1000; Cell Signaling catalog no. 2118L) in 5% milk PBS-T for 2 h. The membranes were washed three times with PBS-T and incubated with goat or donkey anti-rabbit 800IRDye (1:10,000; Li-Cor) in 5% milk PBS-T for 1 h. The membranes were washed four times with PBS-T before being imaged using a Li-Cor Odyssey IR imaging system.

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions reveals a novel protrusion length-matching mechanism
    Article Snippet: Tissue sections and fixed cells were imaged using a Nikon A1R laserscanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488 and 561 excitation LASERS, a 100x/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. Structure illumination microscopy was performed using an Applied Precision DeltaVision OMX (GE Healthcare) equipped with a 60x Plan-Apochromat N/1.42 NA oil immersion objective (Olympus) and processed using softWorx software (GE Healthcare).

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions and function
    Article Snippet: Tissue sections and fixed cells were imaged using a Nikon A1R laser-scanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488 and 561 excitation LASERS, a 100x/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. Structure illumination microscopy was performed using an Applied Precision DeltaVision OMX (GE Healthcare) equipped with a 60x Plan-Apochromat N/1.42 NA oil immersion objective (Olympus) and processed using softWorx software (GE Healthcare).

    Light Microscopy:

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions reveals a novel protrusion length-matching mechanism
    Article Snippet: The tissue sections and fixed cells were imaged using a Nikon A1R laser-scanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488- and 561-nm excitation LASERS, a 100×/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. The membranes were blocked for 1 h in 5% milk in PBS containing 0.1% Tween 20 (PBS-T), washed once with PBS-T, and then incubated with primary antibodies against MYO7B (1:100; Sigma catalog no. HPA039131), CDHR2 (1:100; Sigma catalog no. HPA012569), CDHR5 (1:500; Sigma catalog no. HPA009081), ANKS4B (1:200; Sigma catalog no. HPA043523), USH1C (1:250; Sigma catalog no. HPA027398), or GAPDH (1:1000; Cell Signaling catalog no. 2118L) in 5% milk PBS-T for 2 h. The membranes were washed three times with PBS-T and incubated with goat or donkey anti-rabbit 800IRDye (1:10,000; Li-Cor) in 5% milk PBS-T for 1 h. The membranes were washed four times with PBS-T before being imaged using a Li-Cor Odyssey IR imaging system.

    Article Title: Single Molecule Spectroscopy Studies of Acid-Base Chemical Gradients Using Nile Red as a Probe of Local Surface Acidity.
    Article Snippet: .. Dye-doped aminosilane gradient films were imaged on a wide-field epifluorescence microscope that has been described previously.36 Briefly, this microscope is built upon a Nikon TiE inverted light microscope that employs the Nikon Perfect Focus stabilization system. .. Nile Red fluorescence was excited by 0.5 mW (measured prior to entry into the microscope) of 532 nm laser light derived from a solid-state laser (Coherent Sapphire, 50 mW).

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5.
    Article Snippet: .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions reveals a novel protrusion length-matching mechanism
    Article Snippet: Tissue sections and fixed cells were imaged using a Nikon A1R laserscanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488 and 561 excitation LASERS, a 100x/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. Structure illumination microscopy was performed using an Applied Precision DeltaVision OMX (GE Healthcare) equipped with a 60x Plan-Apochromat N/1.42 NA oil immersion objective (Olympus) and processed using softWorx software (GE Healthcare).

    Article Title: A heterologous in-cell assay for investigating intermicrovillar adhesion complex interactions and function
    Article Snippet: Tissue sections and fixed cells were imaged using a Nikon A1R laser-scanning confocal microscope. .. TIRF live cell imaging was performed on a Nikon TiE inverted light microscope equipped with 488 and 561 excitation LASERS, a 100x/1.49 NA TIRF objective, and a Photometrics Evolve EM-CCD camera. .. Structure illumination microscopy was performed using an Applied Precision DeltaVision OMX (GE Healthcare) equipped with a 60x Plan-Apochromat N/1.42 NA oil immersion objective (Olympus) and processed using softWorx software (GE Healthcare).

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5
    Article Snippet: Sections were incubated with primary antibodies overnight at 4°C, then incubated with secondary antibodies and counterstained with DAPI (#D1306, Invitrogen TM , Waltham, MA) for 2 h at RT (see antibody information in Supplemental Tables S2 and S3 ). .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5
    Article Snippet: Sections were incubated with primary antibodies overnight at 4°C, then incubated with secondary antibodies and counterstained with DAPI (#D1306, Invitrogen TM , Waltham, MA) for 2 h at RT (see antibody information in Table S1&2). .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..

    Microscopy:

    Article Title: Single Molecule Spectroscopy Studies of Acid-Base Chemical Gradients Using Nile Red as a Probe of Local Surface Acidity.
    Article Snippet: .. Dye-doped aminosilane gradient films were imaged on a wide-field epifluorescence microscope that has been described previously.36 Briefly, this microscope is built upon a Nikon TiE inverted light microscope that employs the Nikon Perfect Focus stabilization system. .. Nile Red fluorescence was excited by 0.5 mW (measured prior to entry into the microscope) of 532 nm laser light derived from a solid-state laser (Coherent Sapphire, 50 mW).

    Imaging:

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5.
    Article Snippet: .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5
    Article Snippet: Sections were incubated with primary antibodies overnight at 4°C, then incubated with secondary antibodies and counterstained with DAPI (#D1306, Invitrogen TM , Waltham, MA) for 2 h at RT (see antibody information in Supplemental Tables S2 and S3 ). .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..

    Article Title: Characterization of the intraspecies chimeric mouse brain at embryonic day 12.5
    Article Snippet: Sections were incubated with primary antibodies overnight at 4°C, then incubated with secondary antibodies and counterstained with DAPI (#D1306, Invitrogen TM , Waltham, MA) for 2 h at RT (see antibody information in Table S1&2). .. All immunohistological imaging was performed on a Nikon TiE inverted light microscope using a SPECTRA3 light source (Lumencor), a 20X Plan Apo (NA 0.75) objective, and a Hamamatsu ORCA-Flash4.0 V2 camera. ..



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